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Biblioteca(s): |
Epagri-Sede. |
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Data corrente: |
18/03/2015 |
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Data da última atualização: |
18/03/2015 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
PASSOS, J. F. M.; OLIVEIRA, A. M. R.; COSTA, P. B.; PASSAGLIA, L. M. P. |
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Título: |
Antagonistic ability of bacteria isolated from apple trees against Colletotrichum gloeosporioides. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE GENÉTCIA, 59., 2013, Águas de Lindóia, SP. Resumos... Ribeirão Preto, SP: SBG- Sociedade Brasileira de Genética, 2013. |
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Idioma: |
Inglês |
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Conteúdo: |
Apple is one of the most important fruit crops consumed in the world. Its culture covers almost the whole region of the temperate climate in the Northern and Southern hemispheres. Currently, the conventional production system adopted for this culture depends on non-renewable resources, such as fertilizers, in addition to the excessive amount of insecticides. This system is being replaced by an organic system. Several bacterial strains termed ?plant growth promoting rhizobacteria? (PGPR) have been used to increase yield and quality of crops. These bacteria also play an important role in biological control, as they can induce systemic resistance against various pathogens. This study aims to evaluate the diversity of cultivable PGPR isolated from rhizospheric soil and roots of apple trees cultivated under different crop systems in the south of Santa Catarina state. Samples of roots and rhizospheric soil of apple trees cultivated in: Native Field (NF), Orchard Organic (OO), and Orchard Conventional (OC) were collected, together with soil samples from an area never used for agriculture. Bacteria were identified at the genus level by PCR-RFLP 16S rRNA gene analysis and partial sequencing methodologies and were evaluated for their ability to produce indolic compounds and siderophores and to solubilize phosphates. The antagonistic ability of these bacteria against Colletotrichum gloeosporioides, the causal agent of leaf spot disease, was also evaluated. Among the 300 strains isolated, 214 were able to produce siderophores, 34 were able to solubilize phosphates and 21 isolates produced more than 100 mg ml-1 of indolic compounds in in vitro assays. A seventy six isolates presented some antagonist activity against C. gloeosporioides, probably due to antibiotic production. Among the most abundant genera identified were, respectively, Rahnella sp., Enterobacter sp., Pseudomonas sp., Stenotrophomonas sp., Rhizobium sp., Pantoea sp., and Raoultella sp. An in vivo experiment was conducted in a greenhouse and isolate 89 (identified as Microbacterium schleiferi) was able to reduce the damage of the phytopathogenic fungus. Seven days after fungus exposition plants inoculated with isolate 89 presented, on average, only 30% of their leaf area affected, whereas plants without bacterial treatment presented between 60 to 73% of their leaf area affected by fungus attack. This result is very promising for agronomical purposes since it will give to producer?s time to prevent further fungal damages. MenosApple is one of the most important fruit crops consumed in the world. Its culture covers almost the whole region of the temperate climate in the Northern and Southern hemispheres. Currently, the conventional production system adopted for this culture depends on non-renewable resources, such as fertilizers, in addition to the excessive amount of insecticides. This system is being replaced by an organic system. Several bacterial strains termed ?plant growth promoting rhizobacteria? (PGPR) have been used to increase yield and quality of crops. These bacteria also play an important role in biological control, as they can induce systemic resistance against various pathogens. This study aims to evaluate the diversity of cultivable PGPR isolated from rhizospheric soil and roots of apple trees cultivated under different crop systems in the south of Santa Catarina state. Samples of roots and rhizospheric soil of apple trees cultivated in: Native Field (NF), Orchard Organic (OO), and Orchard Conventional (OC) were collected, together with soil samples from an area never used for agriculture. Bacteria were identified at the genus level by PCR-RFLP 16S rRNA gene analysis and partial sequencing methodologies and were evaluated for their ability to produce indolic compounds and siderophores and to solubilize phosphates. The antagonistic ability of these bacteria against Colletotrichum gloeosporioides, the causal agent of leaf spot disease, was also evaluated. Among the 300 strains isolate... Mostrar Tudo |
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Palavras-Chave: |
antagonism; Apple tree; Colletotrichum gloeosporioides; ISR; PGPR. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 03257naa a2200217 a 4500
001 1123375
005 2015-03-18
008 2013 bl uuuu u00u1 u #d
100 1 $aPASSOS, J. F. M.
245 $aAntagonistic ability of bacteria isolated from apple trees against Colletotrichum gloeosporioides.$h[electronic resource]
260 $c2013
520 $aApple is one of the most important fruit crops consumed in the world. Its culture covers almost the whole region of the temperate climate in the Northern and Southern hemispheres. Currently, the conventional production system adopted for this culture depends on non-renewable resources, such as fertilizers, in addition to the excessive amount of insecticides. This system is being replaced by an organic system. Several bacterial strains termed ?plant growth promoting rhizobacteria? (PGPR) have been used to increase yield and quality of crops. These bacteria also play an important role in biological control, as they can induce systemic resistance against various pathogens. This study aims to evaluate the diversity of cultivable PGPR isolated from rhizospheric soil and roots of apple trees cultivated under different crop systems in the south of Santa Catarina state. Samples of roots and rhizospheric soil of apple trees cultivated in: Native Field (NF), Orchard Organic (OO), and Orchard Conventional (OC) were collected, together with soil samples from an area never used for agriculture. Bacteria were identified at the genus level by PCR-RFLP 16S rRNA gene analysis and partial sequencing methodologies and were evaluated for their ability to produce indolic compounds and siderophores and to solubilize phosphates. The antagonistic ability of these bacteria against Colletotrichum gloeosporioides, the causal agent of leaf spot disease, was also evaluated. Among the 300 strains isolated, 214 were able to produce siderophores, 34 were able to solubilize phosphates and 21 isolates produced more than 100 mg ml-1 of indolic compounds in in vitro assays. A seventy six isolates presented some antagonist activity against C. gloeosporioides, probably due to antibiotic production. Among the most abundant genera identified were, respectively, Rahnella sp., Enterobacter sp., Pseudomonas sp., Stenotrophomonas sp., Rhizobium sp., Pantoea sp., and Raoultella sp. An in vivo experiment was conducted in a greenhouse and isolate 89 (identified as Microbacterium schleiferi) was able to reduce the damage of the phytopathogenic fungus. Seven days after fungus exposition plants inoculated with isolate 89 presented, on average, only 30% of their leaf area affected, whereas plants without bacterial treatment presented between 60 to 73% of their leaf area affected by fungus attack. This result is very promising for agronomical purposes since it will give to producer?s time to prevent further fungal damages.
653 $aantagonism
653 $aApple tree
653 $aColletotrichum gloeosporioides
653 $aISR
653 $aPGPR
700 1 $aOLIVEIRA, A. M. R.
700 1 $aCOSTA, P. B.
700 1 $aPASSAGLIA, L. M. P.
773 $tIn: CONGRESSO BRASILEIRO DE GENÉTCIA, 59., 2013, Águas de Lindóia, SP. Resumos... Ribeirão Preto, SP: SBG- Sociedade Brasileira de Genética, 2013.
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